以人为本 Proteome Project (HPP) was launched in 2010 after successful completion of 以人为本 Genome Project (HGP) to identify, characterise and map 人 proteome (the entire set of proteins expressed by the 人 genome). On its tenth anniversary, HPP has released the first high-stringency blueprint that covers 90.4% of the 人 proteome. As the code of life, this milestone has very significant implications for 人 health and therapeutics.
在2003中完成, 以人为本 Genome Project (HGP) was an international collaboration set up in 1990 with an aim to identify the complete set of 人 genes and to determine the complete sequence of DNA bases in the 人 genome. On January 15, 2001, HGP had released initial sequence and analysis of the 人 genome. Identifying, characterizing and mapping of 人 proteome (entire complement of proteins coded by the genome) was the next logical step. Therefore, 以人为本 Proteome Organization (HUPO) was formed on February 9, 2001 to promote proteomics research. On September 23, 2010 HUPO officially launched 以人为本 Proteome Project (HPP) with an aim to prepare a blueprint of the 人 蛋白质组 (1).
的分析 人 基因组 predicts around 20,300 protein-coding genes. The entire set of proteins coded by these genes constitute the ‘人 proteome’. 以人为本 proteome is much larger than ‘human genome’ because one gene can be expressed in range of forms (proteoforms) as a result of chemical modifications during and after translation. It is estimated that a million proteoforms may coexist in a single individual. In 2010, at the start of HPP, barely 70% of the proteins predicted by the genome analysis were identified. The agenda of the proteome project was to fill this knowledge gap. With the advances in technology, it has become possible to detect and quantify proteins and their forms with higher precision. Still, there are a good number of missing proteins (蛋白质 由基因组分析预测,但仍未检测到) (2,3). 该项目仍在进行中; 然而,已经达到了一个里程碑。
16年2020月90.4日,在成立十周年之际,HPP发布了首个覆盖XNUMX%人类蛋白质组的高严格蓝图 (1). 这极大地提高了我们对人类生物学的了解以及对细胞和分子水平分子机制的理解,特别是人类蛋白质组所发挥的作用,直接导致癌症、心血管和传染病的诊断和治疗研究和开发,特别是针对个性化和精准医疗 (4).
人类的发展 蛋白质 Atlas 在人类诊断和治疗领域的进一步研究方面取得了非常重大的进展 (5,6).
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参考文献:
- HUPO 2021。蛋白质组学时间表。 可在 https://hupo.org/Proteomics-Timeline.
- neXtProt 2021。人类蛋白质组。 可在线获取 https://www.nextprot.org/about/human-proteome 30 年 2020 月 XNUMX 日访问。
- Inserm,2020 年。蛋白质组学:90% 以上的生命密码被翻译。 07 年 2020 月 XNUMX 日发布。可在线获取: https://www.inserm.fr/actualites-et-evenements/actualites/proteomique-code-vie-traduit-plus-90 30 年 2020 月 XNUMX 日访问。
- Adhikari, S., Nice, EC, Deutsch, EW 等。 2020. 人类蛋白质组的高严格蓝图。 发表时间:16 年 2020 月 11 日。 Nature Communication 5301, 2020 (XNUMX)。 DOI: https://doi.org/10.1038/s41467-020-19045-9
- Digre A. 和 Lindskog C.,2020 年。人类蛋白质图谱——人类蛋白质组在健康和疾病中的空间定位。 蛋白质科学第 30 卷,第 1 期。首次出版时间:04 年 2020 月 XNUMX 日。DOI: https://doi.org/10.1002/pro.3987
- 2020 年人类蛋白质图谱。 人类蛋白质图谱可在线获取: https://www.proteinatlas.org/about 30 年 2020 月 XNUMX 日访问。
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